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biotin peg2 nhs ester  (Tokyo Chemical Industry)

 
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    Tokyo Chemical Industry biotin peg2 nhs ester
    Biotin Peg2 Nhs Ester, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotin+peg2+nhs+ester/bdp+ester+fl+nhs/pm42086734-236-4-6
    Average 86 stars, based on 1 article reviews
    biotin peg2 nhs ester - by Bioz Stars, 2026-11
    86/100 stars

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    Labeling:

    Article Title: KLP-6 is a kinesin superfamily protein resistant to ADP inhibition.
    Article Snippet: .. Tubulin was labeled with Biotin-PEG2-NHS ester (Tokyo Chemical Industry, Tokyo, Japan) and AZDye647 NHS ester (Fluoroprobes, Scottsdale, AZ, USA) as described 61. ..

    Article Title: Modular photostable fluorescent DNA blocks dissect the effects of pathogenic mutant kinesin on collective transport
    Article Snippet: .. Tubulin was purified from porcine brain as described.48 Tubulin was labeled with biotin-PEG2-NHS ester (Tokyo Chemical Industry, Tokyo, Japan) and AZDye647 NHS ester (Fluoroprobes, Scottsdale, AZ, USA) as described.49 To polymerize Taxol-stabilized microtubules labeled with biotin and AZDye647, 30 mM unlabeled tubulin, 1.5 mM biotin-labeled tubulin, and 1.5 mM AZDye647-labeled tubulin were mixed in BRB80 buffer (80 mM PIPES, 1 mM MgCl2, and 1 mM EGTA) supplemented with 1 mM GTP and incubated for 15 min at 37 C. Then, an equal amount of BRB80 supplemented with 40 mM Taxol was added and further incubated for more than 15 min. ..

    Purification:

    Article Title: Modular photostable fluorescent DNA blocks dissect the effects of pathogenic mutant kinesin on collective transport
    Article Snippet: .. Tubulin was purified from porcine brain as described.48 Tubulin was labeled with biotin-PEG2-NHS ester (Tokyo Chemical Industry, Tokyo, Japan) and AZDye647 NHS ester (Fluoroprobes, Scottsdale, AZ, USA) as described.49 To polymerize Taxol-stabilized microtubules labeled with biotin and AZDye647, 30 mM unlabeled tubulin, 1.5 mM biotin-labeled tubulin, and 1.5 mM AZDye647-labeled tubulin were mixed in BRB80 buffer (80 mM PIPES, 1 mM MgCl2, and 1 mM EGTA) supplemented with 1 mM GTP and incubated for 15 min at 37 C. Then, an equal amount of BRB80 supplemented with 40 mM Taxol was added and further incubated for more than 15 min. ..

    Incubation:

    Article Title: Modular photostable fluorescent DNA blocks dissect the effects of pathogenic mutant kinesin on collective transport
    Article Snippet: .. Tubulin was purified from porcine brain as described.48 Tubulin was labeled with biotin-PEG2-NHS ester (Tokyo Chemical Industry, Tokyo, Japan) and AZDye647 NHS ester (Fluoroprobes, Scottsdale, AZ, USA) as described.49 To polymerize Taxol-stabilized microtubules labeled with biotin and AZDye647, 30 mM unlabeled tubulin, 1.5 mM biotin-labeled tubulin, and 1.5 mM AZDye647-labeled tubulin were mixed in BRB80 buffer (80 mM PIPES, 1 mM MgCl2, and 1 mM EGTA) supplemented with 1 mM GTP and incubated for 15 min at 37 C. Then, an equal amount of BRB80 supplemented with 40 mM Taxol was added and further incubated for more than 15 min. ..



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell chemical biology

    Article Title: Inhibition of Flaviviruses by Targeting a Conserved Pocket on the Viral Envelope Protein

    doi: 10.1016/j.chembiol.2018.05.011

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: 4 nmol of ZIKV sE protein was mixed with 20 nmol of biotin-PEG2-NHS (9-biotinlamino-4,7-dioxanonanoic acid N -hydroxysuccinimidyl ester, Broadpharm) in 1X PBS to which 20 mM NaHCO 3 , pH 8.3 was added to adjust the solution to slightly basic pH.

    Techniques: Recombinant, Luciferase, Amplified Luminescent Proximity Homogenous Assay, Software